polyclonal antibody anti ten 2  (R&D Systems)

Journal: Frontiers in Neuroscience

Article Title: A Putative Role of Teneurin-2 and Its Related Proteins in Astrocytes

doi: 10.3389/fnins.2019.00655

Figure Lengend Snippet: LPHN-LI reactive astrocytes. Confocal microscopy photomicrographs showing reactive astrocytes (GFAP-LI) stained with nuclear DAPI and exhibiting immunoreactivity to LPHN-1 (A–A”) , LPHN-2 (B–B”) , and LPHN-3 (C–C”) in cerebral cortex with mechanical brain lesion in cerebral cortex from 3-day postoperative period group. Note that nearly all reactive astrocytes exhibited substantial immunoreactivity to LPHN-1 (A-A”) , moderate to LPHN-3 (C–C”) and modest to LPHN-2 (B–B”) (arrows). Ab1 (LPHN-1), omission of LPHN-1 primary polyclonal antibody; GFAP-LI, glial fibrillary acidic protein-like immunoreactive; LPHN-1-LI, latrophilin-1-like immunoreactive; LPHN-2-LI, latrophilin-2-like immunoreactive; LPHN-3-LI, latrophilin-3-like immunoreactive; * , cortical lesion area.

Article Snippet: Additionally, an adsorption test was done using Ten-2 primary polyclonal antibody and the control peptide (Ten-2, Lot # E1011, sc-165674P, N-13, 100 μg/0.5 mL, Santa Cruz Biotechnology, CA, USA).

Techniques: Confocal Microscopy, Staining